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Amazon is ending its hybrid work policy and ordering staff back to the office five days a week.
The change will go into effect in January, boss Andy Jassy said in a memo to staff announcing the new rule.
He said he believed the move would help staff be "better set up to invent, collaborate, and be connected enough to each other".
Mr Jassy has long been known as a sceptic of remote work, but Amazon staff were previously allowed to work from home two days a week.
The company's push to get its corporate staff back into the office has been a source of tension within the company, which employs more than 1.5 million people globally in full- and part-time positions, including hundreds of thousands in office roles.
Staff at its Seattle headquarters staged a protest last year as the company tightened the full remote work allowance that was put in place during the pandemic.
Amazon subsequently fired the organiser of that protest, prompting claims of unfair retaliation, a dispute that has been taken up with labour officials.
In his message on Monday, Mr Jassy said he was worried that Amazon - which has long prided itself on preserving the intensity of a start-up while growing to become a tech giant - was seeing its corporate culture diluted by flexible work and too many bureaucratic layers.
He said he had created a "bureacracy mailbox" for staff to make complaints about unnecessary rules and the company was asking managers to reorganise so that managers are overseeing 15% more people.
Amazon said those changes could lead to job cuts, which would be communicated at the team level.
In addition to returning to the office five days a week, the company would bring back "assigned desk arrangements in locations that were previously organized that way" including its US headquarters.