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Police in Canada's capital are telling protesters to "leave the area now" as the demonstration against Covid restrictions drags on.
Hundreds remain in Ottawa in defiance of the newly deployed Emergencies Act.
The warning came as the last remaining border blockade, at Emerson, Manitoba, came to an end on Wednesday.
Authorities already cleared border blockades in Coutts, Alberta, in Surrey, British Columbia and the crucial Ambassador Bridge in Windsor.
Prime Minister Justin Trudeau has taken the unprecedented step of invoking emergency powers to crack down on the demonstrations.
On Wednesday morning, Ottawa police issued leaflets warning those still in the downtown core after nearly three weeks of demonstrations that blocking the streets - or assisting those doing so - face arrest.
"The people of Ottawa are being denied the lawful use, enjoyment and operation of their property and you are causing businesses to close. That is mischief under the criminal code," the leaflets said.
Many Ottawa residents have been angered by the protests, which have affected local businesses and daily life for those living in the city. The police chief resigned this week after mounting criticism over his force's handling of the protests.
The new powers invoked by Mr Trudeau will be used to ban gatherings in the parts of Ottawa most affected by the protest - around Parliament Hill and nearby government buildings and war monuments - and prohibit travel to these areas.
Demonstrators will also be barred from bringing minors to the Ottawa protest.
The powers will also be used to compel the provision of essential services, namely tow truck drivers and companies to help remove vehicles from Ottawa's congested downtown.
The prime minister has maintained he has no plans to deploy the military.
On Wednesday, Public Safety Minister Marco Mendocino said it was essential that "progress be maintained" after the successful clearing of border crossings, where millions of dollars of trade with the US was held up.
Meanwhile, four protesters arrested in connection with a border blockade in Coutts have been charged with conspiring to murder Royal Canadian Mounted Police, as well as mischief by impeding the lawful use of property and weapons charges.
Seven other protesters - one as young as 18 - were arrested and charged with allegations of mischief and a weapons charge.