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Ethiopian officials say they have found the graves of at least 119 civilians after rebel forces withdrew from a village they had occupied.
Tigray's rebels forces have denied any responsibility, calling the accusations "fabricated".
One local official in the Amhara region which borders Tigray described the dead as "innocent farmers, priests, children and women".
The BBC cannot independently verify the allegations.
There is currently a communications blackout in the region, which has been devastated by a 10-month conflict between the rebel Tigray People's Liberation Front (TPLF) and government forces.
Amhara official Ato Sewnet Wubalem said the bodies had been reburied at a church compound.
Local officials and witnesses who spoke to the BBC said rebel fighters committed the atrocity after losing territory during 10 days of heavy fighting in the farming village of Chena.
The TPLF say they have no reported history of targeting civilians, and called for an investigation.
An Amhara administrator told Reuters news agency the number of dead bodies might be higher than initial figures show.
Residents said some people are still missing.
One local hospital representative told the AFP news agency that doctors were still treating wounded people.
"We have received close to 35, 36 civilians but I am not sure they are all from the Chena massacre. Most of them have gun wounds," said Ashenafi Tazebew.
He said that some of the injured "are asking to go to the funerals" of dead family members, even though they need treatment.
The Chena incident is not the first time allegations of innocent civilian deaths have been reported in the Tigray civil war.
Both the national military and Tigray rebels have previously been accused of atrocities including rape and mass civilian killings.
In July the United Nations warned that 400,000 people were living in famine-like conditions as a result of the violence.
The TPLF was the governing party in Tigray, until federal forces ousted them in November 2020 after they seized a military barracks.
The Ethiopian government has designated the group a terrorist organisation - a label which they reject.