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The GMB union has withdrawn its bid for recognition at Amazon's Coventry warehouse, accusing the shopping giant of "dirty tricks".
The union claimed it had surpassed the number of members needed to secure recognition at the site - but that Amazon had taken on 1,000 extra staff to scupper the bid.
Amazon denied the accusation, saying it regularly recruited new staff.
It came as workers at the warehouse announced three more days of strikes.
Staff will walk out from 12 to 14 June as part of a long-running dispute over pay.
The GMB has been battling for the right to officially represent Amazon workers in Coventry for a decade.
A successful bid would force Amazon to negotiate with workers over pay, holidays and sick pay.
Workers at the site had staged the first strike at Amazon's operations in Britain in January, and followed it up with walkouts in February, March and April.