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MK Dons will host rivals AFC Wimbledon as one of 40 ties in the FA Cup first round.
Elsewhere, League One Northampton Town will play sixth-tier neighbours Kettering Town.
The lowest ranked side left in the competition, Hednesford Town, could face Gainsborough Trinity or Boston United at home - but they must win Tuesday's fourth-round qualifying replay against Gateshead first.
Seventh-tier side Harborough Town, making an appearance in round one for the first time, will be looking to extend their run after being drawn at sixth-tier Tonbridge Angels.
The ties will take place over the weekend of 2 and 3 November.
MK Dons and AFC Wimbledon, who are both in League Two, have been rivals since Wimbledon FC relocated to Milton Keynes in 2003 and subsequently changed their name to MK Dons in the summer of 2004.
South London side AFC Wimbledon were formed as a phoenix club and have risen through the divisions in the 22 years since they were founded.
Meanwhile, League One leaders Birmingham City have an away tie against National League side Sutton United and second-placed Wrexham will face League Two's Harrogate Town.
Rotherham United v Cheltenham Town
Barrow v Doncaster Rovers
Worthing v Morecambe
Boreham Wood v Leyton Orient
Exeter City v Barnet
Carlisle United v Wigan Athletic
Tamworth v Huddersfield Town
Hartlepool United or Brackley Town v Braintree Town
Curzon Ashton v Mansfield Town
Wycombe Wanderers v York City
Bradford City v Aldershot Town
Hednesford Town or Gateshead v Gainsborough Trinity or Boston United
Burton Albion v Scarborough Athletic
Tranmere Rovers v Oldham Athletic
Rochdale v Bromley
Walsall v Bolton Wanderers
Grimsby Town v Wealdstone
Bristol Rovers v Weston-Super-Mare
MK Dons v AFC Wimbledon
Altrincham or Solihull Moors v Maidstone United
Stockport County v Forest Green Rovers
Reading v Fleetwood Town
Stevenage v Guiseley
Northampton Town v Kettering Town
Rushall Olympic or Peterborough Sports v Accrington Stanley
Swindon Town v Colchester United
Salford City v Shrewsbury Town
Crewe Alexandra v Dagenham & Redbridge
Port Vale v Barnsley
Chesham United v Lincoln City
Chesterfield v Horsham
Southend United v Charlton Athletic
Notts County v Alfreton Town
Taunton Town or Maidenhead United v Crawley Town
Harrogate Town v Wrexham
Woking v Cambridge United
Gillingham v Blackpool
Tonbridge v Harborough Town
Sutton United v Birmingham City
Newport v Peterborough