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Ambulance workers in the south of England and West Midlands will strike just after the early May bank holiday, the Unite union has said.
The Christie cancer service in Manchester, and hospitals in Birmingham and Lancashire will also be affected.
Unite's general secretary Sharon Graham said the government's pay offer was "not good enough" for her members.
Unite said staff it represents will go on strike on Tuesday 2 May in:
- South Central Ambulance Trust
- South East Coast Ambulance Trust
- West Midlands Ambulance Trust
- The Christie NHS Foundation Trust and Pathology Partnership
- East Lancashire Hospitals NHS Trust
- West Birmingham NHS Trust
The union has already announced strikes on the previous day - 1 May - at the Yorkshire ambulance service and Guy's and St Thomas' NHS Trust in central London.
Unite is the smallest of the three unions which represent ambulance workers, alongside Unison and the GMB. About 2,000 paramedics, call handlers and other staff working for ambulance trusts are expected to walk out across the two days in May.
Thousands of nurses are also expected to strike between 30 April and 2 May in about half of the NHS trusts in England.
The Royal College of Nursing, whose members rejected the government's latest pay offer, said emergency departments, intensive care and cancer services would be affected for the first time.
Members of Unison union, which represents ambulance crews and a smaller number of other staff including nurses, have accepted the same pay deal.
The government offered a 5% pay rise for 2023-24 and a one-off payment of at least £1,655 to top up last year's salary.
The offer covers all NHS staff except doctors.
Ms Graham said: "Unite has been up front and honest that it did not believe that the pay offer was good enough for NHS workers. A lump sum payment and yet another real terms pay cut doesn't meet the challenges faced by NHS workers.
"Where our members have indicated that they want to swiftly return to the picket line, Unite is ensuring they are able to do so."
The government has previously described the pay offer as "fair and generous" and said the decision by nurses to escalate strike action was "hugely concerning for patients".