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Climate change protesters claim to have used superglue, chains and bike locks to block entrances to major insurance marketplace Lloyd's of London.
Dozens of Extinction Rebellion (XR) activists have been at the central London building since about 07:00 BST.
Activists want to stop staff from entering the site, with the aim of closing the business for the day.
Lloyd's of London said it had advised employees to not come to the building but "trading is continuing".
City of London Police said it had sent officers to the building on Lime Street.
XR said it was demanding that Lloyd's stops insuring fossil fuels projects.
The group said some people had scaled the outside of the building. Banners have been unfurled, reading "End fossil fuels now" and "Insure climate justice".
The action comes after activists from Just Stop Oil and XR caused Tower Bridge to be closed for several hours on Friday. Police were called to the bridge after protesters occupied the famous landmark and unveiled a banner.
XR spokesperson Clare Walmsley said of the Lloyd's protest: "We're here to demand an end to all new fossil fuel investments and insurance.
"By underwriting the world's most deadly fossil fuel projects, they are creating climate chaos - floods, famine, wildfires and death.
"Insuring new oil and gas projects in the North Sea will do nothing to solve the cost of living crisis either.
"Instead, it locks us into a system that's already pushing millions into poverty."
In a statement Lloyd's of London said it "supports safe and constructive engagement on climate change and we're continuing to work to support a responsible transition".
It added that employees had been advised not to come to the building "in the interests of our people's safety", but "the market remains open and trading is continuing online".
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